Laboratory challenges with cell-based NIPT

GLOBAL UPDATES (ISPD) Ripudaman Singh1, Niels Uldbjerg2, Lotte Hatt1, Palle Schelde1, Ida Vogel3,4 ARCEDI Biotech ApS Department of Obstetrics and Gynecology, Aarhus University Hospital, Denmark Department of Clinical Genetics, Aarhus University Hospital, Denmark Center for Prenatal Diagnostics, Aarhus University Hospital, Denmark In accordance with ISPD policy, the authors of this article Lotte Hatt, Palle Schelde  Ripudaman Singh disclose […]

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Does Maternal Body Mass Index Affect the Quantity of Circulating Fetal Cells Available to Use for Cell-Based Noninvasive Prenatal Test in High-Risk Pregnancies?

Fetal Diagnosis and Therapy. Sept 2018 Kruckow S, Schelde P, Hatt L, Ravn K, Petersen OB, Uldbjerg N, Vogel I, Singh R. We present the first study that investigates the effect of maternal body mass index (BMI) on the quantity of circulating fetal cells available to use in cell-based noninvasive prenatal test (cbNIPT). cbNIPT has been proposed as a superior alternative to noninvasive prenatal […]

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On the road to replacing invasive testing with cell‐based NIPT: Five clinical cases with aneuploidies, microduplication, unbalanced structural rearrangement, or mosaicism

Prenatal Diagnosis. 2017 Nov;37(11):1120-1124 Vestergaard EM, Singh R, Schelde P, Hatt L, Ravn K, Christensen R, Lildballe DL, Petersen OB, Uldbjerg N, Vogel I Objective: Trophoblastic fetal cells harvested from maternal blood have the capacity to be used for copy number analyses in a cell‐based non‐invasive prenatal test (cbNIPT). Potentially, this will result in increased resolution for detection of subchromosomal aberrations due to high […]

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Fetal cells in maternal blood for prenatal diagnosis: a love story rekindled

Biomark Med. 2017 Jun 15 Singh R, Hatt L, Ravn K, Vogel I, Petersen OB, Uldbjerg N, Schelde P For decades it has been common knowledge that some cells from the developing fetus make their way into maternal circulation, and that this process starts in the early weeks of first trimester. Naturally, when alternative technologies for prenatal diagnosis were being explored, the use of […]

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Genome-wide copy number analysis on DNA from fetal cells isolated from the blood of pregnant women. Prenat Diagn. 2016

Prenat Diagn. 2016 Oct 19 Kølvraa S1, Singh R1, Normand EA2, Qdaisat S2, van den Veyver IB2,3, Jackson L4, Hatt L1, Schelde P1, Uldbjerg N5, Vestergaard EM6, Zhao L2, Chen R2, Shaw CA2, Breman AM2, Beaudet AL2.   Abstract OBJECTIVE: Non-invasive prenatal testing (NIPT) based on fetal cells in maternal blood has the advantage over NIPT based on circulating cell-free fetal DNA in that there is no contamination with maternal DNA. This […]

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A new marker set that identifies fetal cells in maternal circulation with high specificity. Prenat Diagn. 2014. June; 34:1-7.

Prenat Diagn. 2014 Nov;34(11):1066-72 Hatt L1, Brinch M, Singh R, Møller K, Lauridsen RH, Schlütter JM, Uldbjerg N, Christensen B, Kølvraa S. Abstract OBJECTIVE: Fetal cells from the maternal circulation (FCMBs) have the potential to replace cells from amniotic fluid or chorionic villi in a diagnosis of common chromosomal aneuploidies. Good markers for enrichment and identification are lacking. METHOD: Blood samples from 78 normal pregnancies were […]

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Characterization of Fetal Cells from the Maternal Circulation by Microarray Gene Expression Analysis – Could the Extravillous Trophoblasts Be a Target for Future Cell-Based Non-Invasive Prenatal Diagnosis? Fetal Diagn Ther. 2013 Nov 9

Fetal Diagn Ther. 2014;35(3):218-27 Hatt L, Brinch M, Singh R, Møller K, Lauridsen RH, Uldbjerg N, Huppertz B, Christensen B, Kølvraa S. Abstract INTRODUCTION: Circulating fetal cells in maternal blood provide a tool for risk-free, non-invasive prenatal diagnosis. However, fetal cells in the maternal circulation are scarce, and to effectively isolate enough of them for reliable diagnostics, it is crucial to know which fetal cell type(s) […]

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Identification of circulating fetal cell markers by microarray analysis. Prenat Diagn. 2012 Aug;32(8):742-51.

Prenat Diagn. 2012 Aug;32(8):742-51 Brinch M1, Hatt L, Singh R, Møller K, Sommer S, Uldbjerg N, Christensen B, Kølvraa S. Abstract OBJECTIVE: Different fetal cell types have been found in the maternal blood during pregnancy in the past, but fetal cells are scarce, and the proportions of the different cell types are unclear. The objective of the present study was to identify specific fetal cell […]

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