On the road to replacing invasive testing with cell‐based NIPT: Five clinical cases with aneuploidies, microduplication, unbalanced structural rearrangement, or mosaicism
Objective: Trophoblastic fetal cells harvested from maternal blood have the capacity to be used for copy number analyses in a cell‐based non‐invasive prenatal test (cbNIPT). Potentially, this will result in increased resolution for detection of subchromosomal aberrations due to high quality DNA not intermixed with maternal DNA. We present 5 selected clinical cases from first […]read more
Biomark Med. 2017 Jun 15 Singh R, Hatt L, Ravn K, Vogel I, Petersen OB, Uldbjerg N, Schelde P For decades it has been common knowledge that some cells from the developing fetus make their way into maternal circulation, and that this process starts in the early weeks of first trimester. Naturally, when alternative technologies for prenatal diagnosis were being explored, the use of […]read more
Genome-wide copy number analysis on DNA from fetal cells isolated from the blood of pregnant women. Prenat Diagn. 2016
Prenat Diagn. 2016 Oct 19 Kølvraa S1, Singh R1, Normand EA2, Qdaisat S2, van den Veyver IB2,3, Jackson L4, Hatt L1, Schelde P1, Uldbjerg N5, Vestergaard EM6, Zhao L2, Chen R2, Shaw CA2, Breman AM2, Beaudet AL2. Abstract OBJECTIVE: Non-invasive prenatal testing (NIPT) based on fetal cells in maternal blood has the advantage over NIPT based on circulating cell-free fetal DNA in that there is no contamination with maternal DNA. This […]read more
A new marker set that identifies fetal cells in maternal circulation with high specificity. Prenat Diagn. 2014. June; 34:1-7.
Prenat Diagn. 2014 Nov;34(11):1066-72 Hatt L1, Brinch M, Singh R, Møller K, Lauridsen RH, Schlütter JM, Uldbjerg N, Christensen B, Kølvraa S. Abstract OBJECTIVE: Fetal cells from the maternal circulation (FCMBs) have the potential to replace cells from amniotic fluid or chorionic villi in a diagnosis of common chromosomal aneuploidies. Good markers for enrichment and identification are lacking. METHOD: Blood samples from 78 normal pregnancies were […]read more
Characterization of Fetal Cells from the Maternal Circulation by Microarray Gene Expression Analysis – Could the Extravillous Trophoblasts Be a Target for Future Cell-Based Non-Invasive Prenatal Diagnosis? Fetal Diagn Ther. 2013 Nov 9
Fetal Diagn Ther. 2014;35(3):218-27 Hatt L, Brinch M, Singh R, Møller K, Lauridsen RH, Uldbjerg N, Huppertz B, Christensen B, Kølvraa S. Abstract INTRODUCTION: Circulating fetal cells in maternal blood provide a tool for risk-free, non-invasive prenatal diagnosis. However, fetal cells in the maternal circulation are scarce, and to effectively isolate enough of them for reliable diagnostics, it is crucial to know which fetal cell type(s) […]read more
Identification of circulating fetal cell markers by microarray analysis. Prenat Diagn. 2012 Aug;32(8):742-51.
Prenat Diagn. 2012 Aug;32(8):742-51 Brinch M1, Hatt L, Singh R, Møller K, Sommer S, Uldbjerg N, Christensen B, Kølvraa S. Abstract OBJECTIVE: Different fetal cell types have been found in the maternal blood during pregnancy in the past, but fetal cells are scarce, and the proportions of the different cell types are unclear. The objective of the present study was to identify specific fetal cell […]read more